Journal: International Journal of Molecular Sciences

Article Title: Dual Targeting of Stromal Cell Support and Leukemic Cell Growth by a Peptidic PKC Inhibitor Shows Effectiveness against B-ALL

doi: 10.3390/ijms21103705

Figure Lengend Snippet: Cell to cell interaction promotes changes in the expression profile of different adhesion molecules in MSC and B-ALL cell populations. ( A ) A representative experiment showing the expression of VLA-4, VLA-5, VCAM, ICAM-1, CXCR4, and CD44 by flow cytometry in MSC alone or in co-cultures with B-ALL cells stablished for 6 h. ( B ) Unattached B-ALL cells and B-ALL cells recovered from the co-culture were labelled for assessing the expression of the indicated molecules. Data are expressed as mean of MFI ± SEM obtained from three independent experiments.

Article Snippet: The following monoclonal antibodies were used for staining MSC and B-ALL cells, as previously described [ ]: PE mouse anti-human CD49e (clone IIA1, BD Pharmingen, San Jose, CA, USA), APC mouse anti-human CD49d (clone 9F10, BD Pharmingen) or BV711 mouse anti-human CD49d (clone 9F10, BD Horizon) APC mouse anti-human CD54 (clone REA266, Miltenyi Biotec, Auburn, CA, USA), PE mouse anti-human CD106 (VCAM-1) (clone REA269, Miltenyi Biotec) or BV605 mouse anti-human CD106 (Clone 51-10C9, BD Horizon), FITC mouse anti-human CD44 (clone G44-26, BD Pharmingen) or APC mouse anti-human CD44 (clone G44-26) and PE CD184 (CXCR4) (clone 12G5, Miltenyi Biotec).

Techniques: Expressing, Flow Cytometry, Co-Culture Assay

Journal: International Journal of Molecular Sciences

Article Title: Dual Targeting of Stromal Cell Support and Leukemic Cell Growth by a Peptidic PKC Inhibitor Shows Effectiveness against B-ALL

doi: 10.3390/ijms21103705

Figure Lengend Snippet: HKPS treatment reduces the expression of molecules involved in the MSC and B-ALL interaction. ( A ) MSC were pre-treated for 2 h with HKPS (40 μM), STAU (0.5 μM) or vehicle (DMSO 0.4%) and then co-cultures with B-ALL cells were stablished for 6 h. A representative experiment showing the expression of VLA-4, VLA-5, VCAM, ICAM-1, CXCR4, and CD44 evaluated by flow cytometry in CD105+ cell population. MSC alone were used as control. ( B ) Unattached B-ALL cells and B-ALL cells recovered after trypsinization from the co-cultures with MSC were labelled for assessing the expression of the indicated molecules in the CD19+ cell population. Data are expressed as mean of MFI ± SEM obtained from two independent experiments.

Article Snippet: The following monoclonal antibodies were used for staining MSC and B-ALL cells, as previously described [ ]: PE mouse anti-human CD49e (clone IIA1, BD Pharmingen, San Jose, CA, USA), APC mouse anti-human CD49d (clone 9F10, BD Pharmingen) or BV711 mouse anti-human CD49d (clone 9F10, BD Horizon) APC mouse anti-human CD54 (clone REA266, Miltenyi Biotec, Auburn, CA, USA), PE mouse anti-human CD106 (VCAM-1) (clone REA269, Miltenyi Biotec) or BV605 mouse anti-human CD106 (Clone 51-10C9, BD Horizon), FITC mouse anti-human CD44 (clone G44-26, BD Pharmingen) or APC mouse anti-human CD44 (clone G44-26) and PE CD184 (CXCR4) (clone 12G5, Miltenyi Biotec).

Techniques: Expressing, Flow Cytometry, Control

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Computer controlled expansion of equine cord blood mesenchymal stromal cells on microcarriers in 3 L vertical-wheel ® bioreactors

doi: 10.3389/fbioe.2023.1250077

Figure Lengend Snippet: Antibodies used for flow cytometry.

Article Snippet: CD44 , Horse , Bio-Rad , FITC , CVS18 , , MSC.

Techniques: Cytometry

Journal: Cancer cell

Article Title: Liver Cancer Initiation Requires p53 Inhibition by CD44-Enhanced Growth Factor Signaling

doi: 10.1016/j.ccell.2018.05.003

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Antibody details are provided in the . table ft1 table-wrap mode="anchored" t5 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-CD44pan (DF1485) Santa Cruz Biotechnology Cat# SC-7297; RRID: AB_627065 anti-CD44pan (IM7) ABDSerotec (Biorad) Cat# MCA4703; RRID: AB_2076194 anti-CD44v6 ABD Serotec (Biorad) Cat# MCA1967; RRID: AB_323213 anti-AFP R&D Systems Cat# AF5369; RRID: AB_2258018 anti-Ki67 Genetex Cat# GTX16667; RRID: AB_422351 anti-F4/80 (Clone: A3-1) Caltag/Thermo Fisher Cat# MA1-91124; RRID: AB_2277854 anti-Clvd.

Techniques: Recombinant, Blocking Assay, In Situ, TUNEL Assay, Polymer, Plasmid Preparation, Amplification, Staining, Extraction, Labeling, In Situ Hybridization, RNAscope, HD Assay, cDNA Synthesis, SYBR Green Assay, Negative Control, Software