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Image Search Results
Journal: The Journal of Experimental Medicine
Article Title: Peroxisome proliferator–activated receptor δ limits the expansion of pathogenic Th cells during central nervous system autoimmunity
doi: 10.1084/jem.20091663
Figure Lengend Snippet: CD4 + T cells from PPAR-δ −/− mice are hyperresponsive. Naive CD4 + (a) or CD19 + (b) cells were isolated from the spleens of WT or PPAR-δ −/− mice and were stimulated as indicated. Proliferation of these cells was measured using a [ 3 H]-thymidine incorporation assay. Values are means + SEM of triplicate cultures. (c and d) Splenocytes were harvested from nonimmunized WT or PPAR-δ −/− mice. The frequency of CD4 + cells that displayed a memory (CD44 high and CD62L low ; c) or activated (CD69 + or CD25 + ; d) phenotype in each group was determined. In d, values represent mean + SEM frequencies of n = 3–4 mice/group. Data in a–d are representative of two experiments. *, significantly different from WT.
Article Snippet: Antibodies directed against the following mouse cell surface antigens were used: CD4, CD8, CD19, CD11b, CD11c,
Techniques: Isolation, Thymidine Incorporation Assay